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“Depth of field” refers to the thickness of the plane of focus. With a large depth of field, all of the threads can be in focused at the same time. With a smaller / narrower depth of field, only one thread or a part of one thread can be focused, everything else will be out of focus. In order to view the other threads, you must focus downward to view the ones underneath and upward to view the ones that are above.4a) What happens to the depth of field when you increase to a higher magnification (increases, decreases, or remains the same)?4b) Explain how the slide with threads helps you to answer the question above.5) What way does the image move when the slide is moved to the left?6) Describe the orientation of the image you see in the microscope compared to looking at the slide directly. Is it the same?

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