Chat with us, powered by LiveChat SCI250 week 1 Staining Lab and Quiz | acewriters
+1(978)310-4246 credencewriters@gmail.com
  

SCI250 Week 1 Chapter 3 Staining Lab Quiz
Section: Multiple Choice

1. Which of the
following stains is used frequently to identify Mycobacterium and other
bacteria whose cell walls contain high amounts of lipids?
Gram stain
Schaeffer-Fulton stain
Acid-fast stain
Lipidialar stain
Spore Stain
2. Which of the
following stains is used to classify microorganisms based on their cell wall
content?
Capsular stain
Gram stain
Spore stain
Negative stain
Methylene blue
3. In the Gram
stain, Gram-positive bacteria stain with a _________ color due to the
incorporation of the _________ dye into their cell wall.
purple, safranin
purple, crystal violet
red, safranin
red, crystal violet
4. What is the function
of the mordant iodine in a Gram staining procedure?
A mordant causes the crystal violet to run.
A mordant fixes the bacteria to the slide.
A mordant keeps dye attached to an object.
All of the above
5. What is the
order of reagents used in the Gram stain?
crystal violet, iodine, safranin, alcohol
alcohol, crystal violet, iodine, safranin
iodine, crystal violet, safranin, alcohol
crystal violet, iodine, alcohol, safranin
crystal violet, safranin, alcohol, iodine
6. Following a
Gram stain you observe clear, glassy areas inside the bacterial cells. What
does this suggest? Which stain would you use to confirm this?
A simple stain
A flagellar stain
Another Gram stain
An endospore stain
An acid-fast stain
7. The presence of
a capsule around bacterial cells usually indicates their increased
disease-causing potential and resistance to disinfection. Capsules are
generally viewed by ________
spore staining.
scanning electron microscopy.
Gram staining.
Ziehl-Neelsen staining.
negative staining.
Section: True or False

8. Fluorescent
microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
True
False
9. Older, pure
cultures of Gram-positive organisms can appear red when Gram stained.
True
False
Section: Matching

10. Use of two or
more dyes to differentiate among bacterial species or to distinguish various
structures of an organism; for example, the Gram stain.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
11. A technique for
observing flagella by coating the surfaces of flagella with a dye or a metal
such as silver.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stai
Cationic dye (also called basic dye)
12. A differential
stain that uses crystal violet, iodine, alcohol, and safranin to differentiate
bacteria. Positive bacteria stain dark purple; negative ones stain pink or red.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
13. Technique of
staining the background around a specimen, leaving the specimen clear and
unstained.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
14. A differential
stain used to make endospores easier to visualize.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
15. A single dye used
to reveal basic cell shapes and arrangements.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
16. A differential
stain for organisms that are not decolorized in alcohol, such as the bacteria
that cause Hansen’s Disease (leprosy) and tuberculosis.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
17. An ionic
compound, used for staining bacteria, in which the positive ion imparts the
color.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)

error: Content is protected !!